This Manifesto calls for the widespread implementation of Comprehensive Sexuality Education (CSE) as a fundamental human right, fostering empowerment, inclusivity, and respect for all individuals. Developed through a collaborative process involving experts, organizations, and individuals, it outlines key principles to guide the integration of CSE globally, rooted in evidence-based practices and human rights. We urge policymakers, educators, healthcare professionals, and all stakeholders to join in advocating for universal access to quality CSE.